Kinetic Chromogenic TAL Assay

The prepared test solution is mixed with the reconstituted main reagent in a microplate well

Endotoxin activates Factor C, initiating an enzymatic cascade that culminates in the activation of a clotting enzyme.

The enzyme hydrolyzes a specific chromogenic substrate, releasing p-nitroaniline (pNA).

The increase in absorbance at 405 nm is measured kinetically over 90 minutes at 37°C.

The reaction onset time is determined, and the endotoxin concentration in the test solution is calculated by interpolation from a standard curve.