| Catalogue | DNK-1410-1 |
| Method | Colorimetric |
| Specification | 96 tests |
| Sample | Dialysate/ Reverse Osmosis Water/ Replacement Fluid |
| Storage | 2-8 ℃ |
The pre-treated samples are added into the Main Reagent which contains Factor C and Factor B.
Factor C is activated by LPS and activated C activate Factor B, activated Factor B converts proclotting enzyme to clotting enzyme.
The clotting enzyme hydrolyzes the substrate (Boc-leu-Gly-Arg-PNA) to release PNA.
The absorbance is measured at 405nm kinetically. The concentration of LPS is interpreted according to a standard curve.
The ET Assay is working on a microplate reader with kinetic reading at 405nm and temperature control at 37℃.
Recommended microplate readers are Tecan Sunrise or BioTek ELX808iu, with incubator and kinetic reading at 405nm.