Dynamiker Fungus (1-3)-β-D-Glucan Assay

The pre-treated sera are added to the main reagent containing Factor G. Factor G is activated by (1-3)-β-D-glucan and the activated Factor G converts the proclotting enzyme to the clotting enzyme.

The clotting enzyme hydrolyses the substrate(Boc-leu-Gly-Arg-PNA) to release PNA.

The absorbance is measured kinetically at 405 nm.

The concentration of (1-3)-B-D-glucan is interpreted from a standard curve.